Simone Lemeer – Chemical- and Phosphoproteomics

13 Mar Simone Lemeer – Chemical- and Phosphoproteomics

Simone Lemeer works on the elucidation of the mechanisms of action of small molecule inhibitors in cancer cells, as well as identification of the intrinsic resistance mechanisms. State of the art, high sensitive, chemical proteomics and phosphoproteomics technology are used to study the dynamic changes in response to small molecule inhibitor treatment.

Highlighted publications

See my citations on Google Scholar

• Ruprecht B1, Koch H2, Medard G2, Mundt M2, Kuster B3, Lemeer S3. Comprehensive and reproducible phosphopeptide enrichment using iron immobilized metal ion affinity chromatography (Fe-IMAC) columns. Mol Cell Proteomics. 2015 Jan;14(1):205-15. PMID: 25394399. [PubMed] [Read by QxMD] Advances in phosphopeptide enrichment methods enable the identification of thousands of phosphopeptides from complex samples. Current offline enrichment approaches using TiO(2), Ti, and Fe immobilized metal ion affinity chromatography (IMAC) material in batch or microtip format are widely used, but they suffer from irreproducibility and compromised selectivity. To address these shortcomings, we revisited the merits of performing phosphopeptide en [...]
• Wilhelm M1, Schlegl J2, Hahne H3, Moghaddas Gholami A3, Lieberenz M4, Savitski MM5, Ziegler E4, Butzmann L4, Gessulat S4, Marx H6, Mathieson T5, Lemeer S6, Schnatbaum K7, Reimer U7, Wenschuh H7, Mollenhauer M8, Slotta-Huspenina J8, Boese JH4, Bantscheff M5, Gerstmair A4, Faerber F4, Kuster B9. Mass-spectrometry-based draft of the human proteome. Nature. 2014 May 29;509(7502):582-7. PMID: 24870543. [PubMed] [Read by QxMD] Proteomes are characterized by large protein-abundance differences, cell-type- and time-dependent expression patterns and post-translational modifications, all of which carry biological information that is not accessible by genomics or transcriptomics. Here we present a mass-spectrometry-based draft of the human proteome and a public, high-performance, in-memory database for real-time analysis of terabytes of big data, called ProteomicsDB. The in [...]
• Hahne H1, Pachl F, Ruprecht B, Maier SK, Klaeger S, Helm D, Médard G, Wilm M, Lemeer S, Kuster B. DMSO enhances electrospray response, boosting sensitivity of proteomic experiments. Nat Methods. 2013 Oct;10(10):989-91. PMID: 23975139. [PubMed] [Read by QxMD] We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost. [...]
• Marx H1, Lemeer S, Schliep JE, Matheron L, Mohammed S, Cox J, Mann M, Heck AJ, Kuster B. A large synthetic peptide and phosphopeptide reference library for mass spectrometry-based proteomics. Nat Biotechnol. 2013 Jun;31(6):557-64. PMID: 23685481. [PubMed] [Read by QxMD] We present a peptide library and data resource of >100,000 synthetic, unmodified peptides and their phosphorylated counterparts with known sequences and phosphorylation sites. Analysis of the library by mass spectrometry yielded a data set that we used to evaluate the merits of different search engines (Mascot and Andromeda) and fragmentation methods (beam-type collision-induced dissociation (HCD) and electron transfer dissociation (ETD)) for [...]
• Lemeer S1, Zörgiebel C, Ruprecht B, Kohl K, Kuster B. Comparing immobilized kinase inhibitors and covalent ATP probes for proteomic profiling of kinase expression and drug selectivity. J Proteome Res. 2013 Apr 5;12(4):1723-31. PMID: 23495751. [PubMed] [Read by QxMD] Kinases are involved in the regulation of many cellular processes and aberrant kinase signaling has been implicated in human disease. As a consequence, kinases are attractive drug targets. Assessing kinase function and drug selectivity in a more physiological context is challenging and often hampered by the generally low expression level of kinases and the extensive post-translation modification in vivo. Kinase drug selectivity screens by chemica [...]

 People working in the Lemeer group

[profilelist pi=”simone-lemeer” orderby=”last_name”]